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Nucleic Acids Research, 1974, Vol. 1, No. 1 141-148
© 1974

Articles

Abnormal maturation of precursor 16S RNA in a ribosomal assembly defective mutant of E.coli

Jean Feunteun*, Roland Rosset, Chantal Ehresmann{dagger}, Patrick Stiegler{dagger} and Peter Fellner**

Centre de Biochlmie et Biologie Moleculaire du CNRS 31 chemin Joseph Aiguier, 13-Marseillee 9e, France

Received November 8, 1973.

The precursor and mature 16S ribosomal RNAs from a novel thermosensitive ribosomal assembly defective mutant of E. coli, in which genetic evidence suggests that the ribosomal protein S4 is altered, have been isolated and characterised by finger-printing methods. The precursor 16S RNA, which is accumulated at 42°, appears to be identical with that present in wild-type strains, and with that previously described by other workers. However, the mature 16S RNA, which is contained in apparently normal functional 30S ribosomal particles synthesised at the growth-permissive temperature of 30°, is incompletely trimmed and has either one or two additional nucleotldes at its 5'-terminus. This might be due either to an accumulation of two late intermediates In the maturation process, or to mis-trimming of the RNA. Both possibilities suggest that the change in the protein S4 is not only responsible for the thermosensitive character of ribosomal assembly in this mutant, but also causes an alteration in the trimming site, affecting Its recognition by the enzyme involved in the maturation.

*Institut de Recherches Scientifiques sur le Cancer, B.P. No. 8, 94800 Villejuif, France

*Institut de Biologle Moleculaire et Cellulaire, Faculte des Sciences, Universite Louis Pasteur de Strasbourg, rue Rene Descartes, 67 Strasbourg, France

**Searle Research Laboratories, Lane End Road, High Wycombe, Bucks., England


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