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Nucleic Acids Research, 1974, Vol. 1, No. 10 1209-1220
© 1974

Articles

The metabolism of tsocytidine in Escherichla coli

J. Doskocil*, A. Holy* and J. Fillp{dagger}

*Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences 166 10 Praha-Dejvlce, Flemingovo 2, Czechoslovakia {dagger}Institute for Research, Application and Production of Radioisotopes 171 04 Praha, Czechoslovakia

Received June 17, 1974.

Intact cells and cell-free extracts of E. coli convert isocytidine to isocytosine and uracil. The radio active label of 5-[3H]isocytidine is incorporated into RNA and, DNA of growing bacteria at a rate equal to about 1.4% of that of cytidine under similar conditions; the radio activity is found in uridylic, cytidylic and 2'-deoxythymidylic acids, while less than 0.4% of incorporated radio active material might be due to possible incorporation of intact isocytidineo Uridinephosphorylase and cytidine deaminase apparently do not participate in the metabolic conversion of isocytidine.

5-[3H]isocytidine was prepared by platinum-catalyzeddehalogenation of 5-bromoisocytidine in the presence of tritium. The 5-bromo derivative was obtained from 2',3'-O- -isopropylideneisocytidine by N-bromsuccinimide brominationfollowed by acidic hydrolysis.

{dagger}Institute for Research, Application and Production of Radioisotopes, 170 04 Praha, Czechoslovakia


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