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Nucleic Acids Research, 1974, Vol. 1, No. 10 1287-1304
© 1974


Articles

Nucleotide sequence determination of bacteriophage T4 glycine transfer ribonucleic acid

Stephen Stahl*, Gary V. Paddock{dagger} and John Abelson

Department of Chemistry, University of California San Diego, La Jolla, California 92037, USA

Received July 31, 1974.

The nucleotide sequence of a T4 tRNA with an anticodon for glycine has been determined using 32P-labeled material from T4-infected cultures of Escherichia coli. The sequence is: pGCGGAUAUCGUAUAAUGmGDAUUACCUCAGACUUCCAAI{psi}ICUGAUGAUGUGAGTIJICGAUUCUCAUUAUCCGCUCCA-OH. The 74 nucleotide sequence canbe arranged in the classic cloverleaf pattern for tRNAs. The anticodon of T4 tRNAGlY is UCC with a possible modification of the U. The tRNA molecule would thus be expected to recognize the glycine codons GGG and GGA. Comparative analysis of tRNAsGly from T2 and T6 indicate that their sequences are identical with that from T4.


*Department of Biochemistry, University of California, Berkeley, California 94720, USA

{dagger}Department of Biology, University of California, Los Angeles, California 90024, USA


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