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Nucleic Acids Research, 1974, Vol. 1, No. 11 1359-1370
© 1974

Articles

Isopyenic centrifugatian of sheared L-cell chromatin in metrizamide gradients

John J. Monahan and Ross H. Hall

Department of Biochemistry, McMaster University Medical Centre Hamilton, Ontario, Canada L8S 4J9

Received June 26, 1974. Isopycnic gradient centrifugation of L-929 cell chromatin a 38% (W/V) metrizamide solution yields two distinct fractions. The fraction banding at a density of 1.24 gm/cm2(H chromatin) contains about 10% of the DNA present in the fraction banding at a density of 1.18 gm/cm2 (L chromatin). Both fractions contain the same proportions of satellite to main band DNA's. Some differences can be seen in the DNA: protein ratios and types of proteins present in the H and L chromatin fractions.


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