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Nucleic Acids Research, 1974, Vol. 1, No. 11 1411-1420
© 1974

Articles

Repeated DNA sequences in fungi

S.K. Dutta

Department of Botany, Howard University Washington, DC 20001, USA

Received August 6, 1974. Several fungal species, representatives of all broad groups like basidiomycetes, ascomycetes and phycomycetes, were examined for the nature of repeated DNA sequences by DNA:DNA reassociation studies using hydroxyapatite chromatography. All of the fungal species tested contained 10–20%, repeated DNA sequences. There are approximately 100–110 copies of repeated DNA sequences of approximately 4 x 107 daltons piece size of each. Repeated DNA sequence homoduplexes showed on average 5°C difference of Te50 (temperature at which 50% duplexes dissociate) values from the corresponding homoduplexes of unfractionated whole DNA. It is suggested that a part of repetitive sequences in fungi constitutes mitochondrial DNA and a part of it constitutes nuclear DNA.


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