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Nucleic Acids Research, 1974, Vol. 1, No. 11 1497-1502
© 1974

Articles

Rifampicin-resistant bacteriophage PBS2 Infection and RNA polymerase in Bacillus subtills

Alan R. Price, Ranald Hitzeman, Joan Frato and Ken Lombardi

Department of Biological Chemistry, University of Michigan Ann Arbor, Michigan 48104, USA

Received August 16, 1974. Bacteriophage PBS2 replication is unaffected by rifampicin and other rifamycin derivatives, which are potent inhibitors of Bacillus subtilis RNA synthesis. Extracts of gently-lysed infected cells contain a DNA-dependent RNA polymerase activity which is specific for uracil-containing PBS2 DNA. The PBS2-induced RNA polymerase is insensitive to rifamycin derivatives which inhibit the host's RNA polymerase.


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