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Nucleic Acids Research, 1974, Vol. 1, No. 11 1539-1548
© 1974

Articles

Isolation of repeated and self-complementary sequences from E.coli DNA

Ann C. Kato, Lorraine Borstad, Murray J. Fraser and David T. Denhardt

Department of Biochemistry, McGill University Montreal, Quebec, Canada H3G 1Y6

Received August 28, 1974. We have used the single-strand specific nuclease from Neurospora crassa and chromatography on methylated albumin-kieselguhr to purify and characterize repeated and self-complementary sequences from Escherichia coli DNA. Approximately 0.5% of the genome renatures spontaneously at zero time and another 2% renatures somewhat more rapidly than the total DNA. The early renaturing DNA has a base composition and a Tm similar to the total DNA and contains on the average 100 base pairs; the self-complementary DNA also has a base composition like E. coli but contains a mean of 170 base pairs. No evidence was obtained for the presence of a highly redundant sequence.


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W. Hahn, D. Pettijohn, and J Van Ness
One strand equivalent of the Escherichia coli genome is transcribed: complexity and abundance classes of mRNA
Science, August 5, 1977; 197(4303): 582 - 585.
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