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Nucleic Acids Research, 1974, Vol. 1, No. 11 1587-1600
© 1974

Articles

Some general methods of preparing affinity columns

P.V. Sundaram

Max-planck-Institut für Experimentelle Medizin, Abteilung Biochemische Pharmakologle 34 Göttingen, Hermann-Rein-Str. 3, Germany

Received September 19, 1974. Some general methods of covalent coupling of nucleotides, especially derivatized nucleotides, polynucleotides and co-factors to insoluble polymers are described in this paper. Wherever necessary individual methods also carry some information on the binding of enzymes to the same polymers to serve as a guide to the efficiency of the coupling methods.


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