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Nucleic Acids Research, 1974, Vol. 1, No. 12 1631-1648
© 1974

Articles

Enzymatic synthesis of deoxyribo-oligonucleotides of defined sequence. Properties of the enzyme*

Shirley Gillam and Michael Smith**

Department of Biochemistry, Faculty of Medicine, University of British Columbia Vancouver 8, British Columbia, Canada **Medical Research Associate of the Medical Research Council Canada

Received May 30, 1974.

A modified purification is described for an enzyme, from Escherichia coli B, which polymerizes deoxyribonucleoside-5' diphosphates. Under appropriate conditions, the enzyme will add a single deoxyribonucleotide residue to a deoxyribo-oligo-nucleotide primer. At all stages, the enzyme activity copurified with the activity which will polymerize adenosine-5' diphosphate (polynucleotide phosphorylase). Studies of heat stability, the effect of various temperatures of reaction and of disc gel electrophoresis failed to provide evidence that the two activities are separable.

* Research supported by the Medical Research Council of Canada


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