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Nucleic Acids Research, 1974, Vol. 1, No. 12 1675-1690
© 1974

Articles

The fractionation of calf thymus DNA by multiple formation of sedimentable complexes with homologous lysine-rlch histone KAP. Derivative melting curves and ultracentrifugatlon in CsCl concentration gradients

A. Plucienniczak, J. Bartkowiak, A. Krzywiec and H. Panusz

Laboratory of Molecular Biology, Scientific Research Centre of the Medical Academy Lodz, Poland

Received September 20, 1974. The process of fractionation of total calf thymus DNA using a step precipitation of DNA by means of increasing concentrations of the homologous histone KAP was investigated. In addition to the known fractions three so far undescribed ones /in thymus/,characterized by buoyant densities in CsCl equal 1.692, 1.706 and 1.728 g/ccm,were identified. Considerable amounts of preparations seriously enriched in individual satellite fractions were obtained. The ability of GC-rich satellite DNAs to form more soluble complexes with histone KAP is suggested as reason for the observed fractionation.


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