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Nucleic Acids Research, 1974, Vol. 1, No. 12 1703-1720
© 1974

Articles

Isolation and chromatographic behaviour of phenylalanine tRNA from barley embryos

D. Labuda, Z. Janowicz, T. Haertle and J. Augustyniak

Institute of Biochemistry of Poznan Universities 61701 Poznan, ul.Fredry 10, Poland

Received October 2, 1974. Two fractions of phenylalanine tRNA (tRNA1Pne and tRNA2Phe) were purified by BD-cellulose and RPC-5 chromatography of crude tRNA isolated from barley embryos. Successive RPC-5 rechromatography runs of tRNA2Phe showed its conversion into more stable tRNA1Phe, suggesting that the two fractions have essentially the same primary structure. Both tRNA1Pne and tRNA2Pne had about the same acceptor activity, but tRNA2Phe was aminoacylated much faster than tRNA1Phe. RPC-5 chromatography of crude aminoacylated tRNA showed higher contents of phe-tRNA2Phe than of phe-tRNA1Phe but the ratio of these two fractions estimated by relative fluorescence Intensity was about 1. Fluorescence spectra of tRNAPne from barley embryos suggest that it contains Y base similar to Yw from wheat tRNAPhe.


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