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Nucleic Acids Research, 1974, Vol. 1, No. 12 1733-1738
© 1974

Articles

Sequence studies of nonradioactive Mycoplasma tRNAPhe with the aid of polynucleotide phosphorylase and polynucleotide kinase

Kwok S. Szeto and Dieter Söll

Department of Molecular Biophysics and Biochemistry, Yale University New Haven, Connecticut 06520, USA

Received October 10, 1974. The known methods of enzymatic phosphorylation with [32P]phosphate of the 3'- or 5'-hydroxyl group of an oligonucleotide have been applied to oligonucleotides derived from Mycoplasma tRNAPhe. The fingerprints obtained by both methods are very similar to each other and to that of uniformly labelled tRNA. The sequence of some oligonucleotides was determined by partial digestion of the 3'-phosphorylated fragment with spleen phosphodiesterase and of the corresponding 5'-phosphorylated fragment with venom phosphodiesterase.


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