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Nucleic Acids Research, 1974, Vol. 1, No. 12 1739-1752
© 1974

Articles

In vitro tnethylation of nascent reovirus mRNA by a virion-associated methyl transferase

Manny Faust* and Stewart Millward

Department of Biochemistry, McGill University Montreal, Canada

Received October 16, 1974.

Chymotrypsin-derived cores, but not virions, catalyze the transfer ofmethyl groups from S-adenosyl methionine (SAM) to nascent mRNA synthesized in vitro by the core polymerase. The reaction requires Mg$$, and is dependenton the presence of all 4 ribonucleoside triphosphates (rNTPs). Methylation proceeds optimally at 51°C. All ten species of mRNA become methylated during transcription and it is estimated that one methyl residue is incorporated per RNA chain. Experiments designed to determine the location of the methylatednucleotide clearly demonstrate that methylation occurs exclusively at the5' ends of nascent mRNA.

* M.Faust is the holder of a Medical Research Council Studentship (Ottawa).


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