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Nucleic Acids Research, 1974, Vol. 1, No. 5 673-688
© 1974

Articles

Mercury(II) binding to s4U in E.coli tRNAVal

Helen R. Sunshine and Stephen J. Lippard

Department of Chemistry, Columbia University New York, NY 10027, USA

Received March 22, 1974. The accessibility of the s4U base in native tRNAVal from E.coli was monitored by studying the binding of various mercurials. The relative binding order HgBr2{small tilde}HgCl2>>CH3HgOAc{small tilde}CH3HgCl{small tilde}PCMB parallels approximately the steric requirements of linear HgX2 or RHgX compounds for SN2 displacement by sulfur, although other factors are operative. Para-chloromercuribenzoate (PCMB) does not bind the thiolated nucleotide unless the tertiary structure of the tRNA is opened up by removal of Mg2+ ions and heating to 400. Under these conditions, equilibrium dialysis measurements using 14C-labeled PCMB showed one binding site (n = 0.93) with an association constant, K1, of 9 x 104M-1.


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