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Nucleic Acids Research, 1974, Vol. 1, No. 6 753-759
© 1974

Articles

‘DNA snapback’ peptides

Robert L. Novak and James Dohnal

Department of Chemistry, DePaul University 1036 West Belden Avenue Chicago, Illinois 60614, USA

Received April 18, 1974. Thermal denaturation studies show that 10–15% of the calf thymus DNA in the heat denatured (Tyr-Gly-Tyr-Gly-Tyr)-DNA complex renatures spontaneously after cooling. The double-strandness of this DNA was verified by its resistance to single-strand Neurospora endonuclease and by its elution profile on hydroxyapatite columns. The renatured DNA isolated by the latter technique was found to contain 56% GC compared to the 41% GC content of the whole thymus DNA. Alternating tryptophanyl-glycyl and histidyl-glycyl peptides also catalyze the same renaturation. A linear correlation was found between the thermal stabilization afforded to the DNA by the various peptides and their ability to "catalyze" DNA strand renaturation.


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