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Nucleic Acids Research, 1974, Vol. 1, No. 6 835-847
© 1974


Articles

Protein synthesis of the sponge Geodia cydonium: Characterization of the system

Werner E.G. Müller, Isabel Müller and Rudolf K. Zahn*

Physiologisch-Chemisches Institut der Johannes Gutenberg Universität 65 Mainz, Johann Joachim Becher Weg 13, West Germany

Received May 17, 1974.

The ribosomal population of the sponge Geodia cydonium has been examined. The monosomes have a sedimentation constant of 80 S, the sizes of the subunits are approximately 60 S and 45 S respectively. The polyribosomes contain up to 40 ribosomal units. Cell free protein synthesizing systems (cell homogenate as well as reconstituted system) have been prepared and characterized with respect to Mg2+, KCl and ATP concentrations, temperature, pH and time course of the reaction. In the cell-free system and in the cellular system the protein biosynthesis is inhibited by chloramphenicol. It is not affected by cycloheximide.


*Laboratory for Marine Molecular Biology Institue Ruder Boskovic, Rovinj Jugoslavia


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