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Nucleic Acids Research, 1974, Vol. 1, No. 8 945-958
© 1974

Articles

Urea perturbation and the reversibility of nucleohistone conformation

Catherine Chang and Hsueh Jei Li

Department of Chemistry, Brooklyn College of the City University of New York Brooklyn, New York 11210, USA

Received June 18, 1974. Urea effect on conformation and thermal stabilities in nucleohistone and NaCl-treated partially dehistonized nucleohistones has been studied by circular dichroism (CD) and thermal denaturation. Urea imposes a CD change at 278nm of DNA base pairs in native and Nad-treated nucleohistones which can be decomposed into two parts: a decrease in Ag278 for histone-free base pairs and an Increase for histone-bound base pairs. The reduction by urea of Ag220 of bound histones is approximately proportional to the increase of Ag278 of histone-bound base pairs. Urea also lowers the melting temperature of base pairs both free and bound by histones. The presence of urea indeed destroys the secondary structure of bound histones, causing changes in the conformation and thermal stabilities of histonebound base pairs in nucleohistone. Such a urea perturbation on nucleohistone conformation is reversible.


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