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Nucleic Acids Research, 1974, Vol. 1, No. 8 999-1006
© 1974

Articles

Proton magnetic resonance studies of ultraviolet-Irradiated apurinic acid

Ronald O. Rahn* and Thomas Schleich**

*Biology Division, Oak Ridge National Laboratory Oak Ridge, Tennessee 37830, USA **Division of Natural Sciences, University of California Santa Cruz, California 95064, USA

Received June 24, 1974. In apurinic acid, a single-stranded polydeoxyribonucleotide easily obtained upon depurination of DNA, the proton resonances arising from thymine and cytosine are readily observable in aqueous solution at 25°C. Two methyl thymine resonances, centered at1.88 ppm and separated by 0.045 ppm, are observed. We attribute the downfield methylresonance to thymines with no pyrimidine nearest neighbors and the upfield methyl resonance to thymines having pyrimidine neighbors in the 3' and/or 5' positions. Upon ultravioletirradiation, the upfield methyl and thymine H-6 resonances decrease in amplitude andtwo methyl resonances appear at 1.63 and 1.52 ppm, corresponding, respectively, tocytosine-thymine and thymine-thymine cyclobutane dimers. Photoreversal eliminatesthese two minor methyl resonances from the pmr spectrum. We conclude that apurinicacid provides a suitable model system for pmr studies of chemically modified pyrimidinebases in DNA.


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