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Nucleic Acids Research, 1974, Vol. 1, No. 9 1099-1120
© 1974

Articles

Analysis of eucaryotic DNAs with a restriction endonuclease from H.influenzae: isolation of "hidden" satellite DNAs

Gérard Roizès

Centre de Recherches de Biochimie Macromoleculaire (CNRS) B. P. 5051, 34033-Montpellier Cedex, France

Received July 2, 1974. The restriction enzymes from H. influenzae have been used to study various eucaryotic DNAs. Fractions resistant to the action of these enzymes have been isolated from rat, mouse and calf DNAs. The method revealed the existence in rat of several components having the properties of satellite DNAs. Mouse DNA was shown to contain a new satellite of buoyant density 1. 704. Therefore, this appears to be a powerfull method for the isolation of "hidden" satellite DNAs.

Calf satellite DNAs {rho} = 1.705 and {rho} = 1.723 were those resistant to the action of the restriction enzymes whilst satellite DNAs {rho} = 1.714 and {rho} = 1.710 gave fragments of discrete lengths, suggesting internal repeat units of 1 500 and 5 000 base pairs respectively.


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