Nucleic Acids Research, 1982, Vol. 10, No. 1 175-182
© 1982
Articles |
Creation of a data base for sequences of ribdsomal nucleic acids and detection of conserved restriction endonucleases sites through computerized processing
Departamento de Biología Celular, Escuela de Bio1ogía de la Universidad Central de Venezuela Apartado 47525 Los Chaguaramos 1041A, Caracas, Venezuela
+To whom all correspondence should be addressed
Received October 19, 1981.
As part of a project pertaining the organization of riboscmal genes in Kinetoplastidae, we have created a data base for published sequences of ribosomal nucleic acids, with information in Spanish. As a first step in their processing, we have written a computer program which introduces the new feature of determining the length of the fragments produced after single or multiple digestion with any of the known restriction enzymes. With this information we have detected conserved SAU 3A sites: (i) at the 5' end of the 5.8S rRNA and at the 3' end of the small subunit rRNA, both included in similar larger sequences; (ii) in the 5.8S rRNA of vertebrates (a second one), which is not present in lower eukaryotes, showing a clear evolutive divergence; and, (iii) at the 5' terminal of the small subunit rRNA, included in a larger conserved sequences. The possible biological importance of these sequences is discussed.
*Present address: Department of Ophtalmology. Mount Sinai School of Medicine of the City University of New York. One Gustave Levy Place. New York. New York 10029. U.S.A.