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Nucleic Acids Research, 1982, Vol. 10, No. 1 295-304
© 1982

Articles

MAXAMIZE. A DNA sequencing strategy advisor

Rene Bach1, Peter Friedland1, Douglas L. Brutlag2 and Larry Kedes3,

1Departments of Computer Science, Stanford University Stanford, CA 94305 USA 2Departments of Biochemistry, Stanford University Stanford, CA 94305 USA 3Departments of Medicine, Stanford University Stanford, CA 94305 USA

Address all correspondence to: Dr. L.H.Kedes, 151M, VA Hospital, Miranda Avenue, Palo Alto, CA 94304, USA

Received September 15, 1981. The MAXAMIZE advisory system determines from user-provided restriction maps an optimal strategy to do nucleotide sequencing by methods involving end-labeled fragments. The maps may be either simple linear restriction maps of fragments or complex circular maps including restriction sites of a vector. The whole system is interactive and is written in the Genetic English language provided by the GENESIS System, a molecular genetics knowledge representation and manipulation package. In addition, MAXAMIZE provides bookkeeping facilities for sequencing and offers advice on how to verify the newly obtained sequence data.


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