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Nucleic Acids Research, 1982, Vol. 10, No. 11 3381-3389
© 1982

MOLECULAR BIOLOGY

Two distinct conformations of rat liver ribosomal 5S RNA

Indrek Toots, Rolf Misselwitz*, Siegfried Böhm*, Heinz Welfle*, Richard Villems and Mart Saarma+

Lab. Molecular Genetics, Inst. Chemical Physics and Biophysics, Estoman Academy of Sciences 14/16 Kingissepa St., 202400 Tartu, Estonian SSR, USSR *Dep. Optic Spectroscopy, Central Inst. Molecular Biology Academy of Sciences of the GDR, 1115 Berlin-Buch, GDR

+To whom correspondence should be addressed

Received March 11, 1982. Revised May 11, 1982. Accepted May 11, 1982.

Three different conformers of rat liver 5S ribosomal RNA were investigated by partial nuclease cleavage technique using S nuclease and cobra venom endoribonuclease (CVE) as conformational probes. Urea-treated and renatured 5S RNA co-migrate on non-denaturing gels, but exhibit distinct differences in their nuclease cleavage patterns. The most prominent differences in S, nuclease and CVE accessibility of these conformers are located in region 30–50 and around nucleotides 70 and 90. The third form of 5S RNA with higher electrophoretic mobility was generated by EDTA treatment. The cleavage patterns of this 5S RNA conformer are similar to that characteristic for the renatured 5S RNA. The results demonstrate the difference in secondary structure and possibly different tertiary base-pairing interactions of 5S RNA conformers.


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