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Nucleic Acids Research, 1982, Vol. 10, No. 11 3427-3444
© 1982

MOLECULAR BIOLOGY

The gene for the large subunit of ribulose-1,5-bisphosphate carboxylase in Euglena gracilis chloroplast DNA: location, polarity, cloning, and evidence for an intervening sequence

Gary L. Stiegler, H.Marshall Matthews, Scott E. Bingham and Richard B. Hallick

Department of Chemistry, University of Colorado Boulder, CO 80309, USA

Received February 26, 1982. Revised May 6, 1982. Accepted May 6, 1982.

The gene for the large subunit (LS) of ribulose-1,5-bisphosphate carboxylase of Euglena gracilis Z chloroplast DNA has been mapped by heterologous hybridization with DNA restriction fragments containing internal sequences from the Zea maya and Chlamydomonas reinhardii LS genes. The Euglena LS gene which has the same polarity as the Euglena rRNA genes has been located with respect to Pst I, Pvu I, and HindIII sites within the Eco RI fragment Eco A. The region of Euglena chloroplast DNA complementary to an 887 bp internal fragment from the Chlamydomonas chloroplast LS gene is interrupted by a 0.5–1.1 kbp non-complementary sequence. This is the first chloroplast protein gene located on the Euglena genome, and the first evidence for an intervening sequence within any chloroplast protein gene.


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