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Nucleic Acids Research, 1982, Vol. 10, No. 11 3541-3560
© 1982

MOLECULAR BIOLOGY

Changes induced in rat liver polysomal polyadenylated RNAs by depletion of circulating glucocorticoids

W. Bajwa, G.D. Birnie* and J.T. Knowler+

Department of Biochemistry, University of Glasgow Glasgow G12 8QQ *The Beatson Institute for Cancer Research Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, UK

+To whom correspondence should be addressed

Received March 26, 1982. Revised May 7, 1982. Accepted May 7, 1982.

The effects of adrenalectomy on the complexity and the relative abundances of rat liver polyadenylated mRNAs have been investigated. The qualitative and quantitative changes induced by adrenalectomy have been measured by hybridisation of polysomal polyadenylated RNAs from the livers of normal and adrenalectomised rats with total cDNAs, fractionated cDNAs, cDNA representing RNAs specific to normal liver, total unique-sequence DNA and unique-sequence DNA complementary to normal liver polysomal RNA. These analyses indicated that, by 14 days after adrenalectomy, the equivalent of about 7000 sequences of average length 2000 nucleotides can no longer be detected in liver polysomes. Many other sequences are decreased in abundance as compared to normal liver, but some abundant sequences become more abundant. Administration of a glucocorticoid hormone (dexamethasone) very rapidly reverses these changes.


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