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Nucleic Acids Research, 1982, Vol. 10, No. 11 3561-3571
© 1982

CHEMISTRY

Length changes in solution accompanying the B-Z transition of poly (dG-m5dC) induced by Co(NH3)63+

Holly Ho Chen, Elliot Charney* and Donald C. Rau*

Department of Chemistry, George Mason University 4400 University Drive, Fairfax, VA 22030 *Laboratory of Chemical Physics, National Institute of Arthritis, Diabetes, and Digestive and Kidney Diseases, National Institutes of Health Building 2, Room B 1-03, Bethesda, MD 20205, USA

Received March 2, 1982. Revised April 21, 1982. Accepted April 21, 1982.

Transient electric dichroism measurements have been used to observe the rotational relaxation times of 145 base pair fragments of poly (dGm5dC) and random sequence DNA in solution. From these the lengths of the fragments are calculated and the interbase pair separation or rise per base pair (RPB) calculated. The observations show that even in low salt, the addition of very low concentrations of trivalent Co(NH3)63+ results in a transition of the dGm polymer from B-form to Z-form with a change in the RPB from 3.4±.06Å to 3.7±.06Å, the latter form defined by the criterion of an inverted circular dichroism spectra similar to that observed at high salt in the absence of Co(NH3)63+. The 145 base pair DNA and poly (dGm5dC) are found to be essentially fully extended rods in low salt (0.22 mM Na+ solutions.


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