Skip Navigation

This Article
Right arrow Print PDF (365K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (25)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Myrnes, B.
Right arrow Articles by Krokan, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Myrnes, B.
Right arrow Articles by Krokan, H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1982, Vol. 10, No. 12 3693-3701
© 1982

MOLECULAR BIOLOGY

Metabolism of dITP in HeLa cell extracts, incorporation into DNA by isolated nuclei and release of hypoxanthine from DNA by a hypoxanthine-DNA glycosylase activity

BjØrnar Myrnes, Per-Henrik Guddal and Hans Krokan

Institute of Medical Biology, University of TromsØ 9001 TromsØ, Norway

Received May 6, 1982. Accepted May 26, 1982.

dITP may be generated from dATP by a slow, nonenzymatic hydrolysis. While [3H]dITP was degraded rapidly to [3H]deoxyinos1ne by HeLa cell nuclear extracts, no net degradation of [3H]dITP was observed in the presence of physiological concentrations of ATP, apparently because the extract contained deoxynucleoside diphosphate kinase activity that regenerated [3H]dITP from [3H]dIDP. Isolated HeLa cell nuclei, as well as partially purified DNA poly-merase a, incorporated [3H]dITP into DNA at 50–60% of the rate of [3H]dGTP incorporation. No rapid release of the Incorporated radioactivity was observed. The molecular weight of nascent DNA containing dIMP residues, however, decreased slightly after prolonged incubation in the presence of EDTA, suggesting that a repair process is initiated in dIMP-containing chromatin. Furthermore, release of free [3H]hypoxanthine from [3H]dIMP-contain1ng DNA was detected after incubation with nuclear extracts in the presence of EDTA, suggesting the presence of hypoxanthine-DNA glycosylase activity in HeLa cell nuclei.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Bacteriol.Home page
B. Budke and A. Kuzminov
Hypoxanthine Incorporation Is Nonmutagenic in Escherichia coli.
J. Bacteriol., September 1, 2006; 188(18): 6553 - 6560.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. H. Chung, J. H. Back, Y. I. Park, and Y. S. Han
Biochemical characterization of a novel hypoxanthine/xanthine dNTP pyrophosphatase from Methanococcus jannaschii
Nucleic Acids Res., July 15, 2001; 29(14): 3099 - 3107.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. Kraltcheva and S. Hardin
Octamer-primed sequencing technology: effects of dNTP supplementation
Nucleic Acids Res., May 15, 2001; 29(10): e48 - e48.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M. R. Valentine and J. Termini
Kinetics of formation of hypoxanthine containing base pairs by HIV-RT: RNA template effects on the base substitution frequencies
Nucleic Acids Res., March 1, 2001; 29(5): 1191 - 1199.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Yao and Y. W. Kow
Interaction of Deoxyinosine 3`-Endonuclease from Escherichia coli with DNA Containing Deoxyinosine
J. Biol. Chem., December 1, 1995; 270(48): 28609 - 28616.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Lin, A. G. McLennan, K. Ying, Z. Wang, S. Gu, H. Jin, C. Wu, W. Liu, Y. Yuan, R. Tang, et al.
Cloning, Expression, and Characterization of a Human Inosine Triphosphate Pyrophosphatase Encoded by the ITPA Gene
J. Biol. Chem., May 25, 2001; 276(22): 18695 - 18701.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.