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Nucleic Acids Research, 1982, Vol. 10, No. 12 3781-3789
© 1982

CHEMISTRY

5,6-Saturated thymine lesions in DNA: production by ultraviolet light or hydrogen peroxide

Bruce Demple* and Stuart Linn

Department of Biochemistry, University of California Berkeley, CA 94720, USA

* Present address: Imperial Cancer Research Fund, Mill Hill Laboratories, Burtonhole Lane, London, NW7 1AD, UK

Received March 30, 1982. Accepted May 25, 1982.

Thymine analogs with saturated 5–6 bonds are important types of DNA damage that are recognized by the DNA N-glyoosylase activity of E. coli endonu-clease III. Seeking agents which could preferentially form 5,6-hydrated thymine residues In duplex DNA both in vivo and In vitro, we exposed purified duplex DNA to 325- or 313-nm light; however, after such exposure pyrlmidine diners greatly predominated over 5,6-hydrated thymine. Hydrogen peroxide, on the other hand, formed significant numbers of endonuclease III-sensitive sites in vitro which were not apurinic/apyrimidinic lesions and thus were likely to be 5,6-hydrated thymines.


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