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Nucleic Acids Research, 1982, Vol. 10, No. 13 3831-3843
© 1982

MOLECULAR BIOLOGY

Nucleotide sequence of a chromosomal rearranged {lambda}2 immunoglobulin gene of mouse

Gillian E. Wu{dagger}, Nusrat Govindji*, Nobumichi Hozumi* and Helios Murialdo{dagger}

{dagger}Department of Medical Genetics, University of Toronto Toronto Ontario M5S 1A8, Canada *Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto 500 Sherbourne Street, Toronto, Ontario M4X 1K9, Canada

Received April 30, 1982. Accepted June 2, 1982.

The rearranged {lambda}2 gene of the mouse plasmacytoma cell line MOPC315 has been cloned and sequenced. A comparison of its sequence with the sequence of the unrearranged (germ-line) V, J and C gene segments shows that the sequences of the V gene segments differ at six positions. The sequence of the J and C gene segments remained unchanged. These results add support to the hypothesis that somatic mutations occur in immunoglobulin genes and that these mutations do not involve the C gene segment. The degree of homology of the elements of the {lambda}2 gene with those of the {lambda}1 gene and C{lambda}3 and C{lambda}4 gene fragments suggest a pathway of evolution by gene duplication of the immunoglobulin {lambda} light chain locus. According to this scheme the original structure V0-J0C0 gave rise to a structure V0-J1C1-J11C11 by duplication of the J0C0 region. A second duplication encompassing the whole region resulted in the present structure: V1-J3C3-J1C1/V2-J2C2-J4C4.


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