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Nucleic Acids Research, 1982, Vol. 10, No. 13 3967-3980
© 1982

MOLECULAR BIOLOGY

The changes in proviral chromatin that accompany morphological variation in avian sarcoma virus-infected rat cells

David J. Chiswell, David A. Gillespie and John A. Wyke

Imperial Cancer Research Fund P.O. Box 123, Lincoln's Inn Fields, London WC2A 3PX, UK

Received February 23, 1982. Revised June 9, 1982. Accepted June 9, 1982.

The clone All of avian sarcoma virus B77-infected Rat-1 cells comprises both morphologically normal and morphologically transformed derivatives. Transformed subclones, in which virus-specific RNA is readily detectable, contain a provirus that is very sensitive to DNase 1 digestion of chromatin, and show DNase 1 hypersensitive sites at the 5' end of the provirus and in 5' flanking cell DNA. Normal subclones with no detectable virus-specific RNA, whether infected cells that have never been transformed or revertants derived from transformed cells, contain a provirus that is far more resistant to DNase 1 digestion. Moreover this provirus lacks hypersensitive sites at Its 5' end, although DNase 1 hypersensitive sites are present in 5' flanking cell DNA. No DNase 1 hypersensitive sites were detected at the 3' end of the pro-virus in either normal or transformed clones. The pattern of cytosine methylation in the proviral restriction sites of the isoschizomers Msp I and Hpa II differed between transformed and revertant clones; the revertants show additional methylation at some CpG doublets.

1Present address: Amersham International Amersham, Bucks., UK


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