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Nucleic Acids Research, 1982, Vol. 10, No. 13 4059-4069
© 1982

CHEMISTRY

Synthesis and physicochemical properties of two analogs of poly(dA): poly(2-aminopurine-9-ß-D-deoxyribonucleotide) and poly 2-amino-deoxyadenylic acid

Karl Heinz Scheit and Hans-Richard Rackwitz*

Max-Planck-Institut für Biophysikalische Chemie, Abteilung Molekulare Biologie, D-34 Göttingen Am Fassberg, Abteilung Virologie, Martinsried, FRG *Max-Planck-Institut für Biochemie Abteilung Virologie, Martinsried, FRG

Received April 20, 1982. Revised June 10, 1982. Accepted June 10, 1982.

Polymerization of chemically synthesized dn2h6ATP and dn2ATP by deoxynucleotidyl transferase from calf thymus furnished poly(dn2h6A) and poly(dn2A) respectively. The synthetic polynucleotides were characterized by spectroscopic, ultracentrifugation and enzymatic methods. In polynucleotide-polynucleotide interaction, poly(dn2h6A) and poly(dn2A) behaved like analogs of poly(dA).


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