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Nucleic Acids Research, 1982, Vol. 10, No. 15 4543-4550
© 1982

MOLECULAR BIOLOGY

Spliced and unspliced virus specific RNA sequences are associated with purified simian virus 40 chromatin

Martin Zenke and Gerhard Sauer

Institute for Virus Research, German Cancer Research Center D-6900 Heidelberg, FRG

Received May 7, 1982. Revised July 5, 1982. Accepted July 5, 1982.

We have analyzed SV40 specific RNA sequences which are associated with purified viral chromatin. Three discrete size classes of large unprocessed transcripts were identified: 4.8, 3.6 and 3.0 kb. Furthermore, two polyadenylated species of 2.6 and 1.6 kb were found, the latter of which representing mature 16 S mRNA. S, analysis revealed both the presence of spliced and unspliced RNA sequences in transcriptionally active SV40 chromatin.


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