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Nucleic Acids Research, 1982, Vol. 10, No. 15 4551-4563
© 1982

MOLECULAR BIOLOGY

Nucleotide sequence of a protamine component CII gene of Salmo gairdnerii

J.C. States, W. Connor, M.A. Wosnick*, J.M. Aiken, L. Gedamu+ and G.H. Dixon

Department of Medical Biochemistry, Faculty of Medicine, Health Sciences Center, The University of Calgary Calgary, Alberta, Canada T2N 4N1

Received May 5, 1982. Accepted June 16, 1982.

We have isolated, using nick-translated cloned protamine cDNA's as probes, several genomic clones containing protamine gene sequences from a Charon 4A library of Eco R1 digested rainbow trout (Salmo gairdnerii) DNA. One clone was chosen for detailed study and the 2.5 kbp Bam HI-Eco R10 restriction fragment containing the gene was subcloned in the piasmid pBR322. A 920 bp Bgl II-Bam HI restriction fragment contains a sequence coding for protamine component C as well as regions 5' and 3' to the mRNA coding portion. Present in the region 5' to the mRNA coding sequence are the promoter associated signals "TATA" box and "CAAT" box. The 5' untranslated region of the mRNA whose length and sequence were not established from the cDNA clones (1) was determined by nuclease mapping and starts within a sequence similar to the "capping signal" found in other genes. The protamine gene for CII contains no introns, a situation common to most histone genes, but, unlike the histone genes does not occur close to other protamine genes in a "cluster".

*Present address: Connaught Research Institute, Building 17, 1755 Steeles Avenue West, Willowdale, Ontario, Canada.

+Present address: Biochemistry Division, Department of Chemistry, The University Calgary, Alberta, Canada T2N 1N4.


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