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Nucleic Acids Research, 1982, Vol. 10, No. 15 4565-4579
© 1982

MOLECULAR BIOLOGY

Condensation of chromatin into chromosomes preserves an open configuration but alters the DNase I hypersensitive cleavage sites of the transcribed gene

M. Tien Kuo{dagger}, Bhanumathi Iyer and Robert J. Schwarz*

Department of Cell Biology, The University of Texas M.D.Anderson Hospital and Tumor Institute at Houston Houston, TX 77030, USA *Department of Cell Biology, Baylor College of Medicine Houston, TX 77030, USA

{dagger}To whom all correspondence should be addressed

Received April 27, 1982. Revised June 21, 1982. Accepted June 21, 1982.

DNase I was used to probe the molecular organization of the chicken ovalbumin (OV) gene and glyceraldehyde 3-phosphate dehydrogenase (GPD) gene in interphase nuclei and in metaphase chromosomes of cultured chicken lympho-blastoid cells (MSB-1 line). The OV gene was not transcribed in this cell line, whereas the GPD gene was constitutively expressed. The GPD gene was more sensitive to DNase I digestion than the OV gene in both interphase nuclei and metaphase chromosomes, as determined by Southern blotting and liquid hybridization techniques. In addition, we observed DNase I hypersensitive sites around the 5' region of the GPD gene. These hypersensitive sites were not always at the same locations between the interphase nuclei and metaphase chromosomes. Our results suggest that chromatin condensation and decondensation during cell cycle alters nuclease hypersensitive cleavage sites.


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