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Nucleic Acids Research, 1982, Vol. 10, No. 16 4831-4843
© 1982

MOLECULAR BIOLOGY

Blotting of RNA onto ion exchange paper allowing subsequent characterization by in situ translation in addition to blot hybridization*

Chris J.M. Saris1, Henk J. Franssen2, Jos H. Heuyerjans, Jet van Eenbergen and Henri P.J. Bloemers

Department of Biochemistry, University of Nijmegen Geert Grooteplein Noord 21, 6525 EZ Nijmegan, The Netherlands

Received July 15, 1982. Accepted July 26, 1982.

We present the preparation of an ion exchange paper which can be used as a solid carrier in the transfer of RNA from gels.

In addition to detection by blot hybridization to specific probes, transferred RNA can be characterized by cell-free translation in situ. Preparation of the paper is simple, inexpensive and reproducible. Examples of applications are shown and possible other applications are discussed.

*Patent pending.

1Present address: Tumor Virology Laboratory, The Salk Institute, San Diego, CA 92138, USA.

2Present address: Department of Molecular Biology, Agricultural University, 6703 BC Wageningen, The Netherlands.


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