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Nucleic Acids Research, 1982, Vol. 10, No. 16 4861-4872
© 1982

MOLECULAR BIOLOGY

The location of the first AUG codons in cowpea mosaic virus RNAs

George Lomonossoff*, Michael Shanks*, Hans D. Matthes+, Mohinder Singh+ and Michael J. Gait+

*Department of Virus Research, John Innes Institute Colney Lane, Norwich NR4 7UH +Laboratory of Molecular Biology, Medical Research Council Centre Hills Road, Cambridge CB2 2QH, UK

Received July 1, 1982. Accepted July 23, 1982.

We have made use of the known sequence of the 5' ends of both CPMV RNAs to synthesise an oligodeoxynucleotide which can prime second-strand DNA synthesis on full-length cDNA copies of both RNAs. By priming synthesis in the presence of dideoxynucleosids triphosphatas, we have determined the positions of the first AUG codons in each RNA. These occur at positions 115 and 207 on M and B RNA respectively. By using a cloned double-stranded DNA fragment derived from near the 5' end of M RNA as a primer additional sequence from the 5' terminal region of M RNA has been obtained.


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