Skip Navigation

This Article
Right arrow Print PDF (927K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (43)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Gennaro, M. L.
Right arrow Articles by Broadbent, D. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gennaro, M. L.
Right arrow Articles by Broadbent, D. A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1982, Vol. 10, No. 16 4883-4890
© 1982

MOLECULAR BIOLOGY

The expression of biologically active cholera toxin in Escherichia coli

Maria L. Gennaro*, Peter J. Greenaway+ and David A. Broadbent{dagger}

*Dep. Bacterioloty and Virology Instituto Superiore di Sanita, 00161 Roma, Italy +Molecular Genetics Lab., PHLS Centre for Applied Microbiology and Research Porton Down, Wilts. SP4 0JG {dagger}Dep. Medical Microbiology, London Sch. Hygiene and Tropical Medicine London WC1E 7HT, UK

Received June 9, 1982. Accepted July 16, 1982.

Chromosomal DNA from Vibrio cholerae El Tor strain 1621 was digested with Hind III and the products fractionated by centrifugatlon through a sucrose gradient. A 15kb fragment containing the toxin gene of V. cholerae was identified by its homology with the heat labile toxin (LT) gene of toxigenic E. coli. This fragment was cloned in E. coli using pATl53 and subsequently characterised by digestion with different restriction endonucleases. Sequences homologous to the LT gene were identified by hybridisation and then sub-cloned using either pATl53 or pACYC184. Expression of the cloned CT gene in E. coli was detected using both cell culture and ELISA assays. One recombinant plasmid coded for the synthesis of an immunologically active but biologically inactive derivative of CT.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Infect. Immun.Home page
W. Liang, S. Wang, F. Yu, L. Zhang, G. Qi, Y. Liu, S. Gao, and B. Kan
Construction and Evaluation of a Safe, Live, Oral Vibrio cholerae Vaccine Candidate, IEM108
Infect. Immun., October 1, 2003; 71(10): 5498 - 5504.
[Abstract] [Full Text] [PDF]

Home page
 J Med MicrobiolHome page
S. CHAKRABORTY, P. GARG, T. RAMAMURTHY, M. THUNGAPATHRA, J.K. GAUTAM, C. KUMAR, S. MAITI, S. YAMASAKI, T. SHIMADA, Y. TAKEDA, et al.
Comparison of antibiogram, virulence genes, ribotypes and DNA fingerprints of Vibrio cholerae of matching serogroups isolated from hospitalised diarrhoea cases and from the environment during 1997-1998 in Calcutta, India
J. Med. Microbiol., October 1, 2001; 50(10): 879 - 888.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
P. Garg, R. K. Nandy, P. Chaudhury, N. R. Chowdhury, K. De, T. Ramamurthy, S. Yamasaki, S. K. Bhattacharya, Y. Takeda, and G. B. Nair
Emergence of Vibrio cholerae O1 Biotype El Tor Serotype Inaba from the Prevailing O1 Ogawa Serotype Strains in India
J. Clin. Microbiol., November 1, 2000; 38(11): 4249 - 4253.
[Abstract] [Full Text]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.