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Nucleic Acids Research, 1982, Vol. 10, No. 16 4901-4912
© 1982

MOLECULAR BIOLOGY

Identification of novel DNA forms in tomato golden mosaic virus infected tissue. Evidence for a two component viral genome

W.D.O. Hamilton, D.M. Bisaro and K.W. Buck

Department of Pure and Applied Biology, Imperial College London SW7 2BB, UK

Received June 8, 1982. Revised July 20, 1982. Accepted July 20, 1982.

Extracts obtained from cells infected with the geminivirus tomato golden mosaic (TGMV) are shown to contain, in addition to viral single-stranded DNA, several novel species of virus-specific single- and double-stranded DNA (as and ds DNA). The results of nuclease studies and electron microscopy suggest that three of the intracellular DNAa are unit-genome length duplexes of closed circular, relaxed circular, and linear form. The remaining ds DNA species are of high molecular weight and appear to be concatamers consisting of two or more unit-length genones. A low molecular weight virus-specific DNA species was also detected.

Restriction endonuclease digestion of unit-length circular ds TGMV DNA resulted in fragments whose combined size is twice the unit-genome length. Thus ds TGMV is composed of two components of nearly identical size but different nucleotide sequence.


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