Skip Navigation

This Article
Right arrow Print PDF (2229K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Protter, A. A.
Right arrow Articles by Williams, D. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Protter, A. A.
Right arrow Articles by Williams, D. L.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1982, Vol. 10, No. 16 4935-4950
© 1982

MOLECULAR BIOLOGY

Isolation and characterization of a cDNA clone specific for avian vitellogenin II

Andrew A. Protter, Sho-Ya Wang, Gregory S. Shelness, Philomena Ostapchuk and David L. Williams

Department of Pharmacological Sciences, Health Sciences Center, State University of New York at Stony Brook Stony Brook, NY 11794, USA

Received May 25, 1982. Revised July 15, 1982. Accepted July 15, 1982.

A clone for vitellogenin, a major avian, estrogen responsive egg yolk protein, was isolated from the cDNA library of estrogen-induced rooster liver. Two forms of plasma vitellogenin, vitellogenin I (VTG I) and vitelliogenin II (VTG II), distinguishable on the basis of their unique partial proteolysis maps, have been characterized and their corresponding hepatic precursor forms identified. We have used this criterion to specifically characterize which vitellogenin protein had been cloned. Partial proteolysis maps of VTG I and VTG II standards, synthesized in vivo, were compared to maps of protein synthesized in vitro using RNA hybrid-selected by the vitellogenin plasmid. Eight major digest fragments were found common to the in vitro synthesized vitellogenin and the VTG II standard while no fragments were observed to correspond to the VTG I map. A restriction map of the VTG II cDNA clone permits comparison to previously described cDNA and genomic vitellogenin clones.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
G. Friedman, A. Ben-Yehuda, Y. Dabach, G. Hollander, S. Babaey, M. Ben-Naim, O. Stein, and Y. Stein
Macrophage Cholesterol Metabolism, Apolipoprotein E, and Scavenger Receptor AI/II mRNA in Atherosclerosis-Susceptible and -Resistant Mice
Arterioscler. Thromb. Vasc. Biol., November 1, 2000; 20(11): 2459 - 2464.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
R. S. Edinger, E. Mambo, and M. I. Evans
Estrogen-Dependent Transcriptional Activation and Vitellogenin Gene Memory
Mol. Endocrinol., December 1, 1997; 11(13): 1985 - 1993.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
S. Hwang, J. Partin, and W. Lennarz
Characterization of a homolog of human bone morphogenetic protein 1 in the embryo of the sea urchin, Strongylocentrotus purpuratus
Development, January 3, 1994; 120(3): 559 - 568.
[Abstract] [PDF]

Home page
 ScienceHome page
A Elbrecht, C. Lazier, A. Protter, and D. Williams
Independent developmental programs for two estrogen-regulated genes
Science, August 10, 1984; 225(4662): 639 - 641.
[Abstract] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.