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Nucleic Acids Research, 1982, Vol. 10, No. 16 4951-4971
© 1982

MOLECULAR BIOLOGY

Sequence requirements for the transcription of the rabbit ß-globin gene in vivo: -80 region

G.C. Grosveld*, A. Rosenthal+ and R.A. Flavell

Laboratory of Gene Structure and Expression, National Institute for Medical Research Mill Hill, London NW7 1AA, UK

Received May 17, 1982. Revised July 21, 1982. Accepted July 21, 1982.

We have performed a detailed analysis of DNA sequences in the –80 region of the rabbit ß-globin gene that are required for transcription. A variety of rabbit ß-globin gene templates deleted at various sites in the –80 region were linked to an SV40-plasmid recombinant and introduced into HeLa cells; under these conditions the rabbit ß-globin gene is expressed fron its own promotor. The results show that the conserved GGCCAATCT sequence is required for efficient transcription in vivo and further identify another sequence in the region from about –81 to –96 which is also required for transcription in vivo.

*Present address: Erasmus University, Rotterdam, Postbox 1738, 3000 DR Rotterdam, The Netherlands.

+Permanent address: Department of Molecular Virology, Hadassah Medical School, P.O.Box 1172, 91 010 Jerusalem, Israel


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