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Nucleic Acids Research, 1982, Vol. 10, No. 18 5503-5515
© 1982

MOLECULAR BIOLOGY

Structure of 1.711b gm/cm3 bovine satellite DNA: evolutionary relationship to satellite I

Elizabeth J. Taparowsky and Susan A. Gerbi

Brown University, Division of Biology and Medicine Providence, RI 02912, USA

Received April 14, 1982. Revised June 19, 1982. Accepted August 19, 1982.

The Eco RI fragments from the 2600 bp repeating unit of 1.711b gm/cm3 bovine satellite DNA were cloned in pBR322. The structure of the repeat unit was determined and compared to bovine satellite I DNA ({rho}CsCl - 1.715 gm/cm3). All of the DNA in the 1402 bp repeat of satellite I is represented in the sequence of the 2600 bp 1.711b gm/cm3 repeat. The difference between the two repeats is due to a 1200 bp piece of DNA (INS) residing in the middle of the 1.711b gm/cm3 repeat. The INS is AT-rich and has some repetitive components; it bears only limited similarity to the structure of eukaryotic transposable elements. We propose that the 1.711b gm/cm3 satellite DNA arose via the amplification of a 1.715 gm/cm3 satellite repeat altered by a 1200 bp insertion of DNA.


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