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Nucleic Acids Research, 1982, Vol. 10, No. 18 5605-5620
© 1982

MOLECULAR BIOLOGY

Improvements to solid phase phosphotriester synthesis of deoxyoligonucleotides

T.P. Patel, T.A. Millican, C.C. Bose, R.C. Titmas, G.A. Mock and M.A.W. Eaton

Department of Chemistry, Celltech Lid., 250 Bath Road Slough, SL1 4DY, Berkshire, UK

Received July 21, 1982. Accepted August 20, 1982.

A solution of benzenesulphonic acid (3%, w/v) in a dimethylformamide and dichloronethane mixture (9:1, v/v) is shown to be a very effective reagent for the detritylation of dioxyoligonuoleotides attached to a solid support. The levels of depurination with this reagent were lower than those observed with other reagents such as trichloroacetic acid. Coupling reactions are described using above ambient temperatures with no detectable increase in side products. Both procedures have been successfully incorporated into an automated system, which can compete with the rate of synthesis by the phosphite approach.


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