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Nucleic Acids Research, 1982, Vol. 10, No. 18 5717-5721
© 1982


MOLECULAR BIOLOGY

Nucleotide sequences of 5S ribosomal RNA from four oomycete and chytrid water molds

William F. Walker and W. Ford Doolittle

Department of Biochemistry, Sir Charles Tupper Building, Dalhousie University Halifax, Nova Scotia, Canada B3H 4H7

Received April 17, 1982. Revised August 16, 1982. Accepted August 16, 1982.

The nucleotide sequences of the 5S rRNAs of the oomycete water molds Saprolegnia ferax and Pythium hydnosporum and of the chytrid water molds Blastocfadiella Simplex and Phlyctochytrium irregulare were determined by chemical and enzymatic partial degradation of 3' and 5' end-labelled molecules, followed by gel sequence analysis. The two oomycete sequences differed in 24 positions and the two chytrid sequences differed in 27 positions. These pairs differed in a mean of 44 positions. The chytrid sequences clearly most resemble the sequence from the zygomycete Phycomyces, while the oomycete sequences appear to be allied with those from protozoa and slime molds.


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