Nucleic Acids Research, 1982, Vol. 10, No. 2 611-630
© 1982
MOLECULAR BIOLOGY |
On immunoglobulin heavy chain gene switching: two 
2b genes are rearranged via switch sequences in MPC-11 cells but only one is expressed
Biochemistry Department and Molecular Biology Graduate Program, SUNY at Stony Brook NY 11794, USA
Received October 21, 1981. Revised November 23, 1981. Accepted November 23, 1981.
During B lymphocyte differentiation, switches in the expression of heavy chain immunoglobulin constant region (CH) genes occur by a novel DNA recombination mechanism. We have investigated the requirements of the CH gene switch by characterizing two rearranged 
2b genes from a 2b producing mouse myeloma (MPC-11). One of the two 2b genes is present in 2-3 copies per cell (2b strong hybridizer) while the other is present in {small tilde}1 copy per cell (2b weak hybridizer). Genomic clones of the 2b strongly hybridizing gene indicate that this is an abortive switch event between the S3 and S2b regions. How ever, clones of the 2b weakly hybridizing gene suggest a functional rearrangement due to the presence of VH, JH and Sµ sequences. The switch-recombination sites of these rearranged 2b genes and those of other CH genes show a high degree of preference for the sequence AGGTTG 5' of either the Sµ donor site or the appropriate CH S acceptor site. AGGTTG and its analogs are rare in the Sµ region, are somewhat prevalent in S
and in the case of Sµ are found 5' of a tandemly repeated DNA sequence (GAGCT, GGGGT) comprising most of Sµ.
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