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Nucleic Acids Research, 1982, Vol. 10, No. 23 7511-7520
© 1982

MOLECULAR BIOLOGY

Nucleotide sequences of tobacco chloroplast genes for elongator tRNAMet and tRNAVal (UAC): the tRNAVal (UAC) gene contains a long intron

Hiroshi Deno, Akira Kato, Kazuo Shinozaki and Masahiro Sughira*

National Institute of Genetics Mishima, Shizuoka-ken 411, Japan

*To whom correspondence should be addressed; Department of Biology, Nagoya University, Chikusa, Nagoya 464, Japan

Received October 20, 1982. Accepted November 11, 1982.

The nucleotide sequences of tobacco chloroplast genes for elongator tRNAMet and tRNAVal(UAC) have been determined. The tRNAVal gene contains a 571 base pairs intron located in the anticodon loop. The tRNAVal gene is transcribed as a 750 bases precursor RNA molecule. Both tRNAs deduced from the DNA sequences show 97% sequence homologies with those of spinach chloroplasts.


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