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Nucleic Acids Research, 1982, Vol. 10, No. 23 7621-7633
© 1982

MOLECULAR BIOLOGY

m-Aminophenylboronate agarose specifically binds capped snRNA and mRNA

Hans-Erich Wilk, Nicolàs Kecskemethy and Klaus P. Schäfer

Ruhr-Universität Bochum, Lehrstuhl Biochemie D-4630 Bochum, FRG

Received September 20, 1982. Revised November 9, 1982. Accepted November 9, 1982.

m-Aminophenylboronate-substituted agarose binds specifically RNA chains carrying a mature 5' cap. The binding occurs most effectively at pH>8 and involves diester formation between the negatively charged tetrahedric boronate group and the cis-diol of the ribose of the cap. The positive charge introduced by the m7G methylation is necessary for efficient binding although two closely spaced cis-diol groups alone (as in the cap analog NADH) are sufficient for binding. Non-capped RNA (like poly(U) and rRNA) or decapped RNA are not bound. It is shown that the matrix can be used for the isolation of capped small nuclear RNA and mRNA.


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