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Nucleic Acids Research, 1982, Vol. 10, No. 23 7635-7642
© 1982

MOLECULAR BIOLOGY

Organization and nucleotide sequence of nuclear 5S rRNA genes in yellow lupin (Lupinus lutens)

Jan Antoni Rafalski1,2, Maciej Wiewiorowski1 and Dieter SÖll2

1Institute of Bioorganic Chemistry, Polish Academy of Sciences PL-61704 Poznan, Noskowskiego 12, Poland, USA 2Department of Molecular Biophysics and Biochemistry, Yak University 260 Whitney Avenue, New Haven, CT 06511, USA

Received September 15, 1982. Revised November 2, 1982. Accepted November 2, 1982.

Genomic blots of yellow lupin (Luplnua luteus) DNA digested with restriction nuoleases and probed with 32p-labelled Luplnus 5S RNA reveal that 5S DNA is organized as tandemly repeated sequenoes of one size class, 342 bp. The DNA is extensively methylated. Two cloned BamHI ribosomal repeats were sequenced, revealing sequence divergence within both the coding and spacer regions.


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