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Nucleic Acids Research, 1982, Vol. 10, No. 23 7677-7688
© 1982

MOLECULAR BIOLOGY

Sea urchin small nuclear RNA genes are organized in distinct tandemhy repeating units

Charles O. Card, Gilbert F. Morris, David T. Brown and William F. Marzluff

Department of Chemistry, Florida State University Tallahassee, FL 32306, USA

Received September 7, 1982. Revised October 12, 1982. Accepted October 12, 1982.

The genes coding for the two major small nuclear RNAs in the sea urchin are organized in independent tandem repeating units. The small nuclear RNAs, N1 and N2 were purified from gastrula embryos of Lytechinus variegatus. These RNAs are analogous to the U series of RNA in mammalian cells as judged by their identical 5' termini and the sequence homology of the N1 urchin RNA and Ul mouse RNA. These RNAs were polyadenylated with E. Coli adenylate transferase. A 32PO4 labeled copy of each RNA was made with RNA-dependent DNA polymerase. This copy was used to probe the gene organization of these RNAs by hybridizing to restriction enzyme digests of sperm DNA. Each of these RNAs is coded in a tandemly repeated cluster (at least 30 kb) with a repeat length of 1100–1400 bases. The Nl and N2 clusters are distinct. The N1 repeat has been cloned and the repeating organization confirmed with the cloned gene.


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