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Nucleic Acids Research, 1982, Vol. 10, No. 23 7751-7761
© 1982

MOLECULAR BIOLOGY

A switch region inversion contributes to the aberrant rearrangement of a µ. immnnoglobulin heavy chain gene in MPC-11 cells

Robert Greenberg, Rhonda B. Lang, Meryl S. Diamond and Kenneth B. Marcu

Biochemistry Department, State University of New York Stony Brook, NY 11794, USA

Received August 23, 1982. Revised November 1, 1982. Accepted November 1, 1982.

We describe the unique features of an aberrantly rearranged µ immunoglobulin heavy chain gene isolated from MPC-11 cells (a Y 2b producing Balb/c plasmacytana). A novel rearrangement has occurred 1.5 Kb 5' of the MPC-11 µ gene (denoted 18bµ) resulting in the deletion of the majority of the repetitive switch region (Sµ) and 5' flanking DNA including the Joining (JH ) sequences. The remainder (275 bp) of the Sµ repeat has undergone a carplete sequence inversion. DNA sequences 5' of the inverted Sµ sequence do not resemble Variable (VH ), Diversity (D), JH, or their conserved flanking sequences. A DNA sequence localized 5' or the inverted Sy sequence, (p18bµ -1.4) detects a small family of homologous sequences in Balb/c DNA. The 18bµ-1.4 like sequences lack homology to Sµ , exhibit flanking sequence polymorphisms in 5 out of 6 inbred mouse strains and undergo partial or complete deletion in 5 out of 10 plasmacytomas tested. Two 18bµ-1.4 homologous sequences display a higher copy number in C57B1/6, AL/N and CAL9 mouse strains.


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