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Nucleic Acids Research, 1982, Vol. 10, No. 23 7809-7818
© 1982

MOLECULAR BIOLOGY

Human globin {psi}B2 is not a globin-related sequence

Shi-Hsiang Shen and Oliver Smithies

Laboratory of Genetics, University of Wisconsin Madison, WI 53706, USA

Received August 4, 1982. Revised October 29, 1982. Accepted October 29, 1982.

We have determined the complete nucleotide sequence of 3.4 kilobaae pairs of DNA covering the region of the human ß globin gene cluster where a human globin-related sequence {psi}ß2 was thought to lie (Fritsch, Lawn, and Maniatis (1980) Cell 19, 959–972). Analysis of the resulting data reveals no evidence for any globin-related sequences in this region. The region does, however, contain several stretches of poly (dA-dT).

We have confirmed the observations of Fritsch et al. that DNA from the {psi}ß2 region hybridizes to the poly (dA-dT)-tailed human fetal globin cDNA plasmid, pJW151 (Wilson et al., (1978) Nucl. Acids Res. 5, 563–581) under conditions of low stringency, but we find that this hybridization is abolished by the addition of poly(rA). We conclude that {psi}ß2 is not a globin pseudogene, and that the earlier investigators were probably misled by hybridization between the poly (dA-dT) stretches within the {psi}ß2 region and the tails used in constructing the cDNA plasmid.


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