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Nucleic Acids Research, 1982, Vol. 10, No. 24 7947-7957
© 1982

MOLECULAR BIOLOGY

Molecular cloning and characterization of a complete DNA copy of potato spindle tuber viroid RNA

Peter van Wezenbeek*, Pieter Vos, Jacques Van Boom+ and Albert van Kammen§

Department of Molecular Biology, Agricultural University De Dreijen 11, 6703 BC Wageningen, The Netherlands

§To whom correspondence should be addressed

Received October 28, 1982. Accepted November 16, 1982.

Double-stranded cDNA has been synthesized from RNA of a severe strain of potato spindle tuber viroid using a synthetic oligodeoxyribonucleotide as a primer. Upon Cloning in bacteriophage. M13mp9, two recombinant phages were selected to construct a p8R322-derived plasmid containing a complete viroid DNA copy. Elucidation of the nucleotide sequence revealed four differences with the previously establishment sequence of another PSTV strain, three of which were base exchanges and one a deletion.

*Present address: Laboratory of Biochemistry, University of Nijmegen, 6525 EZ Nijmegen, The Netherlands

+Gorlaeus Laboratories, State University of Leiden, P.O. BoX 9502, 2300 RA Leiden The Netherlands


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